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Clone
7F8
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Immunogen
Acetylated KLH
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Antibody s full description
Mouse Anti- Acetylated Lysine Monoclonal IgG1 Antibody, Clone: 7F8: HRP
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Acetyl Lysine Antibody, Acetylated lysine Antibody, Lysine Antibody
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Raised in
Mouse
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Antibody s target
Acetylated Lysine
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Primary research fields
Cell Signaling, Post-translational Modifications, Acetylation
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF, IP, ELISA
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Antibody s reactivity
Species Independent. Bovine albumin and avian histones were tested.
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Antibody s dilutions
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).
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Bibliography
1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang XJ. (2004). Bioessays 26:1076-1087. 4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. 5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.
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Released date
1-Nov-2007
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (SMC-153). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (SMC-153) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of acetylated lysine showing detection of Acetylated Lysine protein using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8 (SMC-153). Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody (SMC-153) at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker. Mouse Anti-Acetylated Lysine Antibody [7F8] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-153) Mouse Anti-Acetylated Lysine Antibody [7F8] used in Western Blot (WB) on acetylated lysine (SMC-153)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
