MMP-13 ELISA Kit
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Catalog number30 518 101
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PricePlease ask
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Size1 Microplate/ 96 determinations
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ELISA categoryELISA Kit
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ELISA detection limits32 -2000 pg/ml MMP13
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ELISA sensitivity7 pg/ml
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ELISA s descriptionThe quantitative assay is based on a sandwich format. A highly specific monoclonal antibody for the activated form of MMP 13 is immobilized on the plate. MMP 13 is bound to the immobilized antibody, other components of the sample are removed by aspiration and washing. The analyte is detected in two steps using a secondary biotin-labeled antibody and a highly polymerized streptavidin-peroxidase conjugate. Any reagent excess is removed by aspiration and washing after each detection step. The amount of peroxidase bound to each well is determined by the addition od TMB Substrate. The reaction is stopped by adding the Stop Solution, and the resultant color is read in a microtiter plate reader at 450 nm. The concentration of activated MMP 13 in a sample is determined by interpolation from the standard curve.
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Research area interestsCancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology
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Package form96 well microplate
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AntigenMMP-13
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Reacts withMMP-13
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Tested applicationsThe system provides a highly sensitive and specific quantitative determination of activated human MMP-13 in serum, synovial fluid and cell culture supernatants.
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SpecificityMMP-13
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CrossreactivityN/A
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Preservative materialsIf applicable mentioned in the product data sheet
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Storage recommendations6 month
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Shipping and storage conditionsice pack
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Long term storage4 °C
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Assay principleSandwich-ELISA
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Included in the standard packagemicroplate, buffers, antibody conjugate,standards
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Not included in the standard packageSpectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water
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Samples to be analyzedbiological fluids, cell culture
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PubMed citationsSee the datasheet
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Technical datasheetContact us to receive the datasheet
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WarningsFor Research Use only.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbolMMP13, MMP14, MMP24, PIRC58, MMP28, SNORD114-13, SNORD115-13, IGLV3-13, IGHV3OR16-13, ERVK-13
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Short nameMMP-13 ELISA Kit
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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Alternative nameMMP-13 Enzyme-linked immunosorbent assay reagent
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Alternative techniqueelisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 13
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Synonyms gene name
- matrix metalloproteinase 13 (collagenase 3)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 14
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Synonyms gene name
- matrix metalloproteinase 14 (membrane-inserted)
- matrix metallopeptidase 14 (membrane-inserted)
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 24
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Synonyms gene name
- matrix metalloproteinase 24 (membrane-inserted)
- matrix metallopeptidase 24 (membrane-inserted)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-08-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 58
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 28
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Synonyms gene name
- matrix metalloproteinase 28
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-01-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-13
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-13
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-13 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 3-13
- immunoglobulin lambda variable 3-13 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-13 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR16-13
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 13
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Synonyms gene name
- endogenous retrovirus group K, member 13
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Synonyms
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GenBank acession
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Locus
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Discovery year2011-05-05
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Pubmed identfication
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data