MMP-2 Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K2017-100
• Price
  Please ask
• Size
  100 assays

• Description
  for screening or characterizing MMP-2 inhibitors
• Summary
  Matrix metalloproteinase-2 (MMP-2) is a zinc-dependent endopeptidase family member that is capable of degrading denatured collagen, collagen type IV as well as other extracellular matrix proteins in normal physiological processes such as embryonic development, tissue remodeling and in disease processes including metastasis, atherosclerosis, cardiac dysfunction etc. MMP-2 is widely expressed in almost all cell types. Like most MMP's, MMP-2 is secreted as inactive pro-protein that is activated when cleaved by extracellular proteases. Additionally, MMP-2 can be activated intracellularly via S-Glutathionylation. BioVision’s MMP-2 Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing potential inhibitors of MMP-2. In this assay, MMP-2 hydrolyzes a FRET-based MMP-2 substrate and releases the quenched fluorescent group, Mca, which can then be detected fluorometrically at Ex/Em = 325/393 nm. A potent, specific MMP-2 inhibitor is also included in the kit.
• Detection Method
  Fluorescence (Ex/Em = 325/393 nm)
• Species Reactivity
  Human
• Applications
  Screening or characterizing MMP-2 inhibitors
• Sample Type
  Inhibitor compounds
• Features Benefits
  Sensitive way for screening, studying and characterizing potential inhibitors of MMP-2., Quick
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Matrix metalloproteinase-2 (MMP-2) is a zinc-dependent endopeptidase family member that is capable of degrading denatured collagen, collagen type IV as well as other extracellular matrix proteins in normal physiological processes such as embryonic development, tissue remodeling and in disease processes including metastasis, atherosclerosis, cardiac dysfunction etc. MMP-2 is widely expressed in almost all cell types. Like most MMP's, MMP-2 is secreted as inactive pro-protein that is activated when cleaved by extracellular proteases. Additionally, MMP-2 can be activated intracellularly via S-Glutathionylation. BioVision’s MMP-2 Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing potential inhibitors of MMP-2. In this assay, MMP-2 hydrolyzes a FRET-based MMP-2 substrate and releases the quenched fluorescent group, Mca, which can then be detected fluorometrically at Ex/Em = 325/393 nm. A potent, specific MMP-2 inhibitor is also included in the kit.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  MMP-2   Inhibitor   Screening   Kit   Fluorometric  
• Gene symbol
  MMP15, MMP14, MMP24, MMP28, MMP2-AS1, MMP17, MMP2, MMP25, MMP16, MIR1289-2
• Short name
  MMP-2 Inhibitor Screening Kit (Fluorometric)
• Alternative name
  MMP-2 suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:7161
• Gene
  MMP15
• Long gene name
  matrix metallopeptidase 15
• Synonyms gene name
  • matrix metalloproteinase 15 (membrane-inserted)
  • matrix metallopeptidase 15 (membrane-inserted)
• Synonyms
  • MT2-MMP
  • MTMMP2
  • SMCP-2
• GenBank acession
  • Z48482
• Locus
  16q21
• Discovery year
  1996-11-13
• Entrez gene record
  4324
• Pubmed identfication
  • 9070935
  • 9119382
• RefSeq identity
  • NM_002428
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000133466

Gene info

• Identity
  HGNC:7160
• Gene
  MMP14
• Long gene name
  matrix metallopeptidase 14
• Synonyms gene name
  • matrix metalloproteinase 14 (membrane-inserted)
  • matrix metallopeptidase 14 (membrane-inserted)
• Synonyms
  • MT1-MMP
• Synonyms name
  • membrane type 1 metalloprotease
  • membrane type 1-matrix metalloproteinase
• Locus
  14q11.2
• Discovery year
  1994-11-20
• Entrez gene record
  4323
• Pubmed identfication
  • 8015608
• RefSeq identity
  • NM_004995
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000028704

Gene info

• Identity
  HGNC:7172
• Gene
  MMP24
• Long gene name
  matrix metallopeptidase 24
• Synonyms gene name
  • matrix metalloproteinase 24 (membrane-inserted)
  • matrix metallopeptidase 24 (membrane-inserted)
• Synonyms
  • MT5-MMP
• Synonyms name
  • membrane-type 5 matrix metalloproteinase
• GenBank acession
  • AF131284
• Locus
  20q11.22
• Discovery year
  1999-08-06
• Entrez gene record
  10893
• Pubmed identfication
  • 10363975
• RefSeq identity
  • NM_006690
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000032330

Gene info

• Identity
  HGNC:14366
• Gene
  MMP28
• Long gene name
  matrix metallopeptidase 28
• Synonyms gene name
  • matrix metalloproteinase 28
• Synonyms
  • MMP-25
  • MM28
  • EPILYSIN
  • MMP-28
• GenBank acession
  • AF315683
• Locus
  17q12
• Discovery year
  2001-01-09
• Entrez gene record
  79148
• Pubmed identfication
  • 11121398
  • 11255011
• RefSeq identity
  • NM_024302
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000188387

Gene info

• Identity
  HGNC:53142
• Gene
  MMP2-AS1
• Long gene name
  MMP2 antisense RNA 1
• Synonyms
  • ENST00000569037
• Locus
  16q12.2
• Discovery year
  2017-02-16
• Entrez gene record
  107984884
• Pubmed identfication
  • 27816668
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:7163
• Gene
  MMP17
• Long gene name
  matrix metallopeptidase 17
• Synonyms gene name
  • matrix metalloproteinase 17 (membrane-inserted)
  • matrix metallopeptidase 17 (membrane-inserted)
• Synonyms
  • MT4-MMP
• GenBank acession
  • X89576
• Locus
  12q24.33
• Discovery year
  1996-11-13
• Entrez gene record
  4326
• Pubmed identfication
  • 9878265
• RefSeq identity
  • NM_016155
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000168050

Gene info

• Identity
  HGNC:7166
• Gene
  MMP2
• Long gene name
  matrix metallopeptidase 2
• Synonyms gene
  • CLG4
  • CLG4A
• Synonyms gene name
  • matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• Synonyms
  • TBE-1
• Locus
  16q12.2
• Discovery year
  1989-05-25
• Entrez gene record
  4313
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000133202

Gene info

• Identity
  HGNC:14246
• Gene
  MMP25
• Long gene name
  matrix metallopeptidase 25
• Synonyms gene
  • MMPL1
  • MMP20
• Synonyms gene name
  • matrix metalloproteinase 25
  • matrix metallopeptidase-like 1
• Synonyms
  • MT6-MMP
• GenBank acession
  • AF145442
• Locus
  16p13.3
• Discovery year
  2000-12-13
• Entrez gene record
  64386
• Pubmed identfication
  • 10628838
  • 10706098
• RefSeq identity
  • NM_022468
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000177475

Gene info

• Identity
  HGNC:7162
• Gene
  MMP16
• Long gene name
  matrix metallopeptidase 16
• Synonyms gene
  • C8orf57
• Synonyms gene name
  • matrix metalloproteinase 16 (membrane-inserted)
  • chromosome 8 open reading frame 57
  • matrix metallopeptidase 16 (membrane-inserted)
• Synonyms
  • MT3-MMP
  • DKFZp761D112
• GenBank acession
  • D85511
• Locus
  8q21.3
• Discovery year
  1996-11-13
• Entrez gene record
  4325
• Pubmed identfication
  • 7559440
• RefSeq identity
  • NM_005941
• Classification
  • M10 matrix metallopeptidases
• VEGA ID
  OTTHUMG00000163769

Gene info

• Identity
  HGNC:35282
• Gene
  MIR1289-2
• Long gene name
  microRNA 1289-2
• Synonyms gene
  • MIRN1289-2
• Synonyms
  • hsa-mir-1289-2
• Locus
  5q31.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302134
• RefSeq identity
  • NR_031621
• Classification
  • MicroRNA MIR1289 family

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