MLH1 (628CT4.6.1) Monoclonal Antibody

• Catalog number
  bsm-51202M
• Price
  Please ask
• Size
  0.1ml

• Long name
  MLH1 (628CT4.6.1) Monoclonal Antibody
• Also known as
  MLH1 (628CT4.6.1) Monoclonal Antibody
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  MLH1
• Specificity
  This is a highly specific antibody against MLH1.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Monoclonal
• Clone number
  628CT4.6.1
• Source
  This MLH1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 458-489 amino acids from the Central region of human MLH1.
• Gene ID number
  4292
• Swiss Prot
  P40692
• Tested Applications
  WB
• Recommended dilutions
  WB(1:100~400)
• Cross reactivity
  Human
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
• Purification method
  Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  FCC2; COCA2; HNPCC; hMLH1; HNPCC2; DNA mismatch repair protein Mlh1; MutL protein homolog 1; MLH1
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  MLH1   628CT4   Monoclonal  
• Gene symbol
  MLH1
• Short name
  Anti-MLH1
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  Immunoglobulin G (IgG)1
• Alternative name
  MLH1 (628CT4.6.1) Monoclonal Antibody
• Alternative technique
  antibodies
• Alternative to gene target
  mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), COCA2 and FCC2 and hMLH1 and HNPCC and HNPCC2, MLH1 and IDBG-24600 and ENSG00000076242 and 4292, structure-specific DNA binding, nuclei, Mlh1 and IDBG-202755 and ENSMUSG00000032498 and 17350, MLH1 and IDBG-644253 and ENSBTAG00000016758 and 533652

Gene info

• Identity
  HGNC:7127
• Gene
  MLH1
• Long gene name
  mutL homolog 1
• Synonyms gene
  • COCA2
• Synonyms gene name
  • mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2)
  • mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Synonyms
  • HNPCC
  • FCC2
  • HNPCC2
• GenBank acession
  • U07418
• Locus
  3p22.2
• Discovery year
  1993-11-24
• Entrez gene record
  4292
• Pubmed identfication
  • 7903889
• RefSeq identity
  • NM_000249
• Classification
  • MutL homologs
• VEGA ID
  OTTHUMG00000130797
• Locus Specific Databases
  • Hereditary Non-Polyposis Colorectal Cancer, HNPCC
  • Global Variome shared LOVD
  • MLH1 database at LOVD-China
  • LOVD - Leiden Open Variation Database
  • LRG_216

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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