Methionine Assay Kit (Fluorometric)

  • Catalog number
    K442-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    The first kit in the market detecting free methionine in a variety of biological samples. Highly sensitive.
  • Summary
    • Detection method- Fluorescence (Ex/Em = 535/587 nm) • Species reactivity- Eurkaryotes • Applications- Estimation of Methionine in various biological samples. • Detection limit/sensitivity: Can detect as little as 0.5 µM of Methionine
  • Detection Method
    Fluorescence (Ex/Em = 535/587 nm)
  • Species Reactivity
    Eukaryotes
  • Applications
    Estimation of Methionine in various biological samples
  • Sample Type
    Serum, plasma, cell/tissue lysate
  • Features Benefits
    • Simple, sensitive and HTP adaptable Protocol • Specific for Methionine without interference from other thiol based amino acids • This kit can detect as little as 0.5 µM of Methionine in a variety of biological samples
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Methionine (Met) is one of the twenty proteogenic amino acids encoded by the genetic code, and one of nine essential amino acids, meaning it cannot be synthesized in vivo and must be obtained from dietary sources. Met is one of two sulfur-containing amino acids (the other being cysteine) and can serve as a precursor in cysteine biosynthesis. S-adenosylmethionine, another product of Met metabolism, is an important co-factor and methyl donor in a number of biological reactions such as methylation and polyamine synthesis. Met is also an intermediate species in the synthesis of phospholipids such as lecithin, and other small precursor molecules including taurine, an amino-sulfonic acid. Oxidation of Met generates methionine sulfoxide, a species whose concentration tends to increase with age and is known to cause protein mis-folding and loss-of-function of numerous enzymes. This version of the amino acid can be restored back to methionine by another enzyme, methionine sulfoxide reductase. Biologically, Met levels can affect important events such as angiogenesis, protein synthesis, and cartilage production. BioVision’s Methionine Assay Kit utilizes an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide. A probe generates fluorometric signal that can be quantified at Ex/Em: 535/587 nm. The assay shows greater than 10-fold specificity for methionine over cysteine and greater than 40-fold selectivity over other standard and non-standard amino acids. After utilization of a proprietary Sample Clean-Up Mix, the method is suitable for use in blood samples, and can detect concentrations as low as 0.5 µM, or 25 pmole Methionine.
  • Test
    Biovision supplies other types of Assays as 1.
  • Gene target
  • Short name
    Methionine Assay Kit (Fluorometric)
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    Methionine test reagent (fluorescence)
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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