Melanoma Cytoplasmic Tumor Lysate
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Catalog number
XBL-10749
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Price
Please ask
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Size
0.1 mg
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Lysates properties
Human melanoma tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human melanoma tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 grades C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated melanoma tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated melanoma tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
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Lysate s origin
Tissue
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State of tissue
Diseased
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Species of the lysate
Human
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Cellular origibn of the lysate
Melanoma
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Disease of the lysate
Tumor
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Localisation of the biopsy
Human Melanoma tissue
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Ug of lysate used by test
Melanoma cytoplasmic protein lysate is for use in Western blotting, 10 ug to 20 ug per lane is recommended for mini gel.
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Positive control in
the lysate is used as positive control in Western Blot
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PH and medium
HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
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Keep at
Store at 2-8 grades C for continuous use. For extended storage, freeze working aliquots at -70 grades C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
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Caution
This product is for research use only.
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Laboratory requirements
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. GENTAUR is not liable for damages or injuries resulting from receipt and/or use of GENTAUR materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs).
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Animal origin
Human
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Dilution
2 mg/mL or more concentrated
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MeSH Data
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Name
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Concept
Scope note:
A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.
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Tree numbers
- E01.370.225.500.383.910
- E01.370.225.500.388.930
- E05.200.500.383.910
- E05.200.500.388.930
- E05.242.383.910
- E05.242.417.500
- E05.337.550.200.800
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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