Medium 199 w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes - For 1L
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Catalog number
PM-M1908/1L
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Price
Please ask
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Size
25X 1L
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Description
Medium 199 w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes - For 1L is used for maintenance of untransformed cells, production of vaccines, viruses and primary explants of epithelial cells.
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Shipping conditions
-20°C or dry ice
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Storage
+ 2 / + 8 ° C
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Shelflife
36 months
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State
Powder
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Chemical description
Mixture composed of inorganic substanced
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Appearance
Physical state at 20 ºC: S ; Appearance: Powdery ; Colour: Cream
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Density at 20 ºC
1698 kg/m³
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Relative density at 20 ºC
1.698
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PH
4.7 ± 0.3
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Osmolality
248 mOsm/kg ± 10%
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Endotoxin
< 1 EU/ml
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Cell growth test
NA
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Composition
NA
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Sterility
Non-sterile
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Personal precautions
Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
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Uses
Used in biotechnology. For professional user/industrial user only.
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Ordering
To order Medium 199 w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes - For 1L, please use the Cat. Nr.PM-M1908/1L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Medium 199 w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes - For 1L
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Additional description
There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Group
media
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Gene target
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Short name
Medium 199 w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM - For 1L
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Technique
Medium, cell culture Media
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Alternative name
Medium 199 w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 millimeter Hepes - to measure 1L
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Alternative technique
cellculture
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Virus
hhv
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MeSH Data
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Name
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Concept
Scope note:
Methods to study reactions or processes taking place in an artificial environment outside the living organism.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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