Medium 199 (E199) w/ Earle's salts and HEPES w/o L-glutamine, D-glucose, and sodium bicarbonate
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Catalog number
MDP01-10LT
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Price
Please ask
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Size
10 L
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Availability
Please contact us to check current availability
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Long description
Medium 199 (E199) w/ Earle's salts and HEPES w/o L-glutamine, D-glucose, and sodium bicarbonate Medium
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Product format
Powder
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Product category
Animal Cell Culture Media
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Product subcategory
Medium 199
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Shipping method
Ambient temperature
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Storage method
Store at 2˚ to 8˚C.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Description
There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Group
media
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Gene target
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Short name
E199
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Technique
Medium, cell culture Media
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Alternative name
Medium 199 (E199) w/ Earle's salts and HEPES w/o L-glutamine, D-glucose, and sodium bicarbonate
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Alternative technique
cellculture
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Virus
hhv
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MeSH Data
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Name
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Concept
Scope note:
Methods to study reactions or processes taking place in an artificial environment outside the living organism.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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