Medium 199 (E199) w/ Earle's salts and HEPES w/o L-glutamine, D-glucose, and sodium bicarbonate

  • Catalog number
    MDP01-10LT
  • Price
    Please ask
  • Size
    10 L
  • Availability
    Please contact us to check current availability
  • Long description
    Medium 199 (E199) w/ Earle's salts and HEPES w/o L-glutamine, D-glucose, and sodium bicarbonate Medium
  • Product format
    Powder
  • Product category
    Animal Cell Culture Media
  • Product subcategory
    Medium 199
  • Shipping method
    Ambient temperature
  • Storage method
    Store at 2˚ to 8˚C.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Description
    There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
  • Group
    media
  • Gene target
  • Short name
    E199
  • Technique
    Medium, cell culture Media
  • Alternative name
    Medium 199 (E199) w/ Earle's salts and HEPES w/o L-glutamine, D-glucose, and sodium bicarbonate
  • Alternative technique
    cellculture
  • Virus
    hhv
MeSH Data
  • Name
  • Concept
    Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
  • Tree numbers
    • E05.481
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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