MCM7 Antibody

• Catalog number
  R30890
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  MCM7
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This MCM7 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P33993
• Purity
  Antigen affinity
• Description
  Minichromosome Maintenance, s. Cerevisiae, homolog of, 7, also called CDC47, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. Petrocca et al.(2008) found that MCM7 and the precursors of microRNAs(miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.
• Immunogen
  An amino acid sequence from the C-terminus of human MCM7 (DEYEELNVWQVNASRTRITFV) was used as the immunogen for this MCM7 antibody.
• Storage
  After reconstitution, the MCM7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MCM7  
• Gene symbol
  MCM7
• Short name
  Anti-MCM7
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to MCM7
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6950
• Gene
  MCM7
• Long gene name
  minichromosome maintenance complex component 7
• Synonyms gene
  • MCM2
• Synonyms gene name
  • minichromosome maintenance deficient (S. cerevisiae) 7
  • MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)
• Synonyms
  • CDC47
  • PPP1R104
• Synonyms name
  • protein phosphatase 1, regulatory subunit 104
• Locus
  7q22.1
• Discovery year
  1994-12-13
• Entrez gene record
  4176
• Pubmed identfication
  • 7842741
• Classification
  • MCM family
  • MicroRNA protein coding host genes
  • minichromosome maintenance 2-7 complex
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000154671

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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