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Target antigen
MBD1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MBD1(366-381aa NQKRQKCRWRQCLQFA), identical to the related mouse sequence.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the MBD1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The MBD1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
MBD1(Methyl-CpG-Binding Domain Protein 1), also known as PCM1 or CXXC3, is a protein that in humans is encoded by the MBD1 gene. Using PCR on a hybrid panel and FISH, Hendrich et al.(1999) mapped the MBD1 gene to chromosome 18q21, 2.1 cM distal to MBD2. Using yeast 2-hybrid analysis, reciprocal immunoprecipitation analysis, and protein pull-down assays, Fujita et al.(2003) showed that MBD1 interacted directly with MCAF. Deletion analysis revealed that the C-terminal transcriptional repressor domain(TRD) of MBD1 interacted with a conserved C-terminal domain of MCAF. Reporter gene assays showed that MCAF increased the repressive function of the isolated TRD of MBD1 against SP1. Chromatin immunoprecipitation analysis revealed that MBD1 linked MCAF to methylated promoters. Uchimura et al.(2006) found that MBD1 was multiply sumoylated in HeLa cells. Sumoylation did not alter the intracellular localization of MBD1 at nuclear foci in C-33A human cervical cancer cells.
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Related articles
1. Fujita, N., Watanabe, S., Ichimura, T., Ohkuma, Y., Chiba, T., Saya, H., Nakao, M. MCAF mediates MBD1-dependent transcriptional repression. Molec. Cell. Biol. 23: 2834-2843, 2003. 2. Hendrich, B., Abbott, C., McQueen, H., Chambers, D., Cross, S., Bird, A. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes. Mammalian Genome 10: 906-912, 1999. 3. Uchimura, Y., Ichimura, T., Uwada, J., Tachibana, T., Sugahara, S., Nakao, M., Saitoh, H. Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. J. Biol. Chem. 281: 23180-23190, 2006.
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Gene Name
MBD1
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Protein Name
Methyl-CpG-binding domain protein 1
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Gene Full Name
methyl-CpG binding domain protein 1
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Synonyms
CXXC 3 antibody|CXXC-type zinc finger protein 3 antibody|CXXC3 antibody|MBD 1 antibody|MBD1 antibody|MBD1_HUMAN antibody|MECP1 COMPLEX antibody|Methyl CpG binding domain protein 1 antibody|Methyl CpG binding domain protein 1 isoform PCM1 antibody|Methyl CpG binding protein MBD1 antibody|Methyl CpG binding protein splice variant 1 antibody|Methyl CpG binding protein splice variant 2 antibody|Methyl CpG binding protein splice variant 3 antibody|Methyl CpG binding protein splice variant 4 antibody|Methyl-CpG-binding domain protein 1 antibody|Methyl-CpG-binding protein MBD1 antibody|PCM 1 antibody|PCM1 antibody|Protein containing methyl CpG binding domain 1 antibody|Protein containing methyl-CpG-binding domain 1 antibody|Regulator of fibroblast growth factor 2(FGF 2) transcription antibody|RFT antibody|The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
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Uniprot ID
Q9UIS9
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
