Phospho-ERK3 Antibody (pS189)
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Catalog number
F53755-0.4ML
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Price
Please ask
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Size
0.4 ml
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Category
Antibody
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Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Phospho-ERK3 (pS189)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
IHC, ELISA
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Recommended dilutions
IHC (Paraffin): 1:50-1:100
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Notes
Titration of the phospho-ERK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This phospho-ERK3 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
Q16659
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Purity
Antigen affinity
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Description
ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
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Immunogen
This phospho-ERK3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS189 of human ERK3.
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Storage
Aliquot the phospho-ERK3 antibody (phospho-S189 and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
MAPK6, MAPK4
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Short name
Anti-Phospho-ERK3 (pS189)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to Phospho-ERK3 (pS189)
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Alternative technique
antibodies
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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