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Target antigen
MAP1
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Clonality
Monoclonal antibody
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Clone
HM-1
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Raised in
mice
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Type of the antibody
IgG1 monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Mouse, rat
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Analyses
WB,IHC-P,IHC-F
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Immunogen
Rat brain microtubule-associated proteins(MAPs).
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the MAP1 Antibody ( HM1)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The MAP1 Antibody ( HM1) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins(MAPs). MAP1 is one of the major neuronal MAPs as well as being the largest(350KD). MAPs include MAP1A, MAP1B, and MAP2. MAP1a is a single-copy gene spanning 10.5 kb. MAP1a coding sequence is contained in five exons. MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain.
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Related articles
1. Fink, J. K.; Jones, S. M.; Esposito, C.; Wilkowski, J. : Human microtubule-associated protein 1a (MAP1A) gene: genomic organization, cDNA sequence, and developmental-and tissue-specific expression. Genomics 35: 577-585, 1996. 2. Ammarback, J. A.; Obar, R. A.; Hughes, S. M.; Vallee, R. B. : MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain. Neuron 7: 129-139, 1991.
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Gene Name
MAP1A
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Protein Name
Microtubule-associated protein 1A
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Gene Full Name
microtubule-associated protein 1A
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Synonyms
MAP1L|MTAP1A
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Uniprot ID
Human(P78559), Mouse(Q9QYR6), Rat(P34926)
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Entrez GeneID
Human(4130), Mouse(17754), Rat(25152)
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps