Mammalian Mitochondria Isolation Kit For Tissue & Cultured Cells

Description

Isolates highly pure, intact & functional mitochondria
Summary

• Useful for mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and protein profiling. • Western blot and ELISA.
Species Reactivity

Mammalian
Applications

• Useful for mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and protein profiling. • Western blot and ELISA.
Sample Type

• Mammalian tissues. • Cultured Cells.
Features Benefits

Simple & rapid method to isolate highly pure, intact and functional mitochondria from tissues and cultured cells.
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

24 months
Background

Mitochondria are the powerhouse of the cells because they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles: an outer membrane and a folded inner membrane called cristae. Isolated mitochondria are the sample of choice to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling. The kit offers two options for the isolation of intact mitochondria. The first option is utilizing a reagent-based method allowing parallel processing of multiple samples. The second option uses traditional dounce homogenization, which provides better mitochondrial yield.
Data Sheet Link
Additional description

6

Gene target

Mammalian Mitochondria Isolation Kit For Tissue Cultured Cells
Short name

Mammalian Mitochondria Isolation Kit For Tissue & Cultured Cells
Technique

tissue, tissues
Alternative name

Mammalian Mitochondria purification reagent to measure Tissue & Cultured Cells
Alternative technique

tissues, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Tissue

cells, tissue

Name

Radioimmunosorbent Test
Concept

Scope note: Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
Tree numbers
- E05.478.566.380.830
- E05.478.566.639.830
- E05.601.470.380.830
- E05.601.470.639.830
Qualifiers

ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data