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Description
Malt Extract, Certified is a culture median product of animal origin, used for the cultivation of fungi, it contains a combination of carbohydrates and different other growth factor.
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Appearance
Cream to brownish yellow Homogenous Free flowing powder
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Solubility
Freely soluble in distilled/purified water, insoluble in alcohol.
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Clarity
1% w/v aqueous solution remains clear without haziness after autoclaving at 15 lbs pressure (121ºC) for 15 minutes.
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Reaction
Reaction of 10% w/v aqueous solution at 25ºC.
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PH
4.80- 5.80
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Total aerobic microbial count cfu gm
By plate method when incubated at 30-35°C for not less than 3 days. Bacterial Count :
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Total Yeast and mould count cfu gm
By plate method when incubated at 20-25°C for not less than 5 days. Yeast & mould Count :
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Test for Pathogens
1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosaNegative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of sample6. Clostridia- Negative in 10 gms of sample
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Indole Test
NA
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Cultural response
Cultural response observed after an incubation at 25-30ºC for 48-72 hours by preparing Malt Extract Agar (M137) using Malt Extract as an ingredient.
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Chemical Analysis
Sodium chloride
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Storage
Store below 30°C.
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Additional description
Pure extracts used in broths or as control for cell culture and Western Blot.