MAGEA3, human recombinant

  • Catalog number
    9245-10
  • Price
    Please ask
  • Size
    10 µg
  • Synonyms
    CT1.3, HIP8, HYPD, MAGE3, MZ2 D
  • Alternative_names
    CT1.3, HIP8, HYPD, MAGE3, MZ2 D
  • Description
    Member of the MAGE gene family that enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases.
  • Recombinant
    Yes
  • Source
    E.coli
  • Purity by SDS PAGE
    ≥90%
  • Assay
    SDS-PAGE
  • Purity
    ≥90%
  • Molecular Weight
    This protein is fused with a 6× His tag at N-terminus and has a calculated MW of 37.1 kDa.
  • Storage Temp
    -20°C
  • Shipping
    Gel pack
  • Shelf Life
    12 months
  • Concentration
    1 mg/ml
  • Appearance
    Liquid
  • Physical form description
    Liquid
  • Reconstitution Instructions
    In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
  • Background Information
    Melanoma-associated antigen 3, also known MAGEA3, is a member of the MAGE gene family, which comprises 12 known genes, of which 6 are expressed in tumors. The MAGE genes were initially isolated from different kinds of tumors, and based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. MAGEA3 is a tumor-specific antigen widely expressed in solid and hematologic malignancies, but not in normal tissues, with the exception of testis and placenta. Therefore, MAGEA3 is an excellent candidate tumor antigen. Recombinant human MAGEA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
  • Amino acid sequence
    MGSSHHHHHH SSGLVPRGSH MGSMPLEQRS QHCKPEEGLE ARGEALGLVG AQAPATEEQE AASSSSTLVE VTLGEVPAAE SPDPPQSPQG ASSLPTTMNY PLWSQSYEDS SNQEEEGPST FPDLESEFQA ALSRKVAELV HFLLLKYRAR EPVTKAEMLG SVVGNWQYFF PVIFSKASSS LQLVFGIELM EVDPIGHLYI FATCLGLSYD GLLGDNQIMP KAGLLIIVLA IIAREGDCAP EEKIWEELSV LEVFEGREDS ILGDPKKLLT QHFVQENYLE YRQVPGSDPA CYEFLWGPRA LVETSYVKVL HHMVKISGGP HISYPPLHEW VLREGEE
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    MAGEA3  
  • Gene symbol
    MAGEA3
  • Short name
    MAGEA3, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    MAGEA3, H. sapiens Rec.
  • Alternative technique
    rec
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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