GM-CSF/Granulocyte-macrophage colony-stimulating factor

  • Catalog number
    E21-K02
  • Price
    Please ask
  • Size
    10ug
  • Shipping_Temperature
    Ambient
  • Product_State
    Lyophilized
  • Formulation_Description
    Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl, pH7.4.
  • Storage
    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Reconstitution
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Purity
    Greater than 95% as determined by reducing SDS-PAGE.
  • Endotoxin
    Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
  • Background
    Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.
  • Alternative Names
    Granulocyte-macrophage colony-stimulating factor;Csf2;GM-CSF;Colony-stimulating factor;Csfgm
  • Expression system
    E.coli
  • Description
    Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Gene
    Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
  • Gene target
    GM-CSF/Granulocyte-macrophage   colony-stimulating   factor  
  • Gene symbol
    CSF2, PPP1R3A, CSF1, CSF3, IL3
  • Short name
    GM-CSF/Granulocyte-macrophage colony-stimulating factor
  • Species
    Mouse
  • Alternative name
    GM-CSF/Granulocyte-macrophage colony-stimulating factor
  • Tissue
    granulocyte
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
    Colony-Forming Units Assay
  • Concept
    Scope note: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
  • Tree numbers
    • E01.370.225.500.383
    • E05.200.500.383
    • E05.242.383
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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Filters
GM CSF Granulocyte macrophage colony stimulating factor
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