mAB-O, Mouse anti-human Abeta protein (3-10) region, oligomer-specific
-
Catalog numberAS13 2715
-
PricePlease ask
-
Size50 µg
-
-
Available ordering formatLyophilized
-
Immunogensynthetic peptide chosen from human Abeta (1-42) protein
-
Raised inMouse
-
ClonalityMonoclonal
-
CloneIgG1, kappa light chain, (clone number 3E5.F8)
-
PurificationAffinity purified
-
How to reconstituteFor reconstitution add 50 µl of sterile water.
-
Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applicationsELISA (ELISA), immunolocalization (IL), dot blot (Dot)
-
Connected productsAS10 932 | Amyloid beta oligomer-specific monoclonal antibody (OMAB) AS13 2716 | mAB-M | human Abeta (3-10) region, monoclonal antibodySecondary antibodies
-
Recommended dilutions for use1 ug/ml (ELISA capture), 10 ug/ml (IL), 1-2 ug/ml (Dot)
-
Molecular weight expected аpparent4.5 kDa
-
Verified reactivityhuman
-
Possible reactivitymouse, bovine, chicken, dog, porcine, rabbit
-
No reactivityno confirmed exceptions from predicted reactivity are currently known
-
Supplementary informationImmunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.
-
ReferencesBrännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
-
Scientific contextAlzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. Recent findings however suggest that smaller oligomeric forms of Abeta, formed in parallel to the amyloid plaques, excert the predominant tissue damaging effect.Specific identification of the oligomeric forms is as a consequence of great interest. Based on a recently published technique a highly oligomer-specific antibody (mAB-O), targeting Abeta oligomers while omitting reactivity towards the monomeric and fibrillar counterpart, has been developed.
-
NotesNone
-
Protein numberRefer to NCBI
-
TAIR numberRefer to NCBI
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Testagisera supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolPIRC49, SNORD115-10, SNORD116-10, IGHV3OR16-10, IGKV2OR2-10, ERVK-10
-
Short namemAB-O, Mouse anti- Abeta protein (3-10) region, oligomer-specific
-
Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, mouses
-
Hostmouse
-
SpeciesHuman, Humans, Mouses
-
Alternative namemonoclonal-O, Mouse Antibody tohuman Abeta protein (3-10) region, oligomer-specific
-
Alternative techniquemurine, antibodies
-
Tissueregion
-
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 49
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 115-10
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 116-10
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 3/OR16-10 (non-functional)
-
Synonyms gene name
- immunoglobulin heavy variable 3/OR16-10
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
Classification
- Immunoglobulin heavy (IGH) orphons
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 2/OR2-10 (pseudogene)
-
Synonyms gene name
- immunoglobulin kappa variable 2/OR2-10
- immunoglobulin kappa variable 2/OR2-10 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
-
Identity
-
Gene
-
Long gene nameendogenous retrovirus group K member 10
-
Synonyms gene name
- endogenous retrovirus group K, member 10
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2011-05-05
-
Entrez gene record
-
Pubmed identfication
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
