M’Fadyean Stain (Polychrome Methylene Blue)

  • Catalog number
    S047-20X500ML
  • Price
    Please ask
  • Size
    20X500ML
  • Description
    M’Fadyean Stain (Polychrome Methylene Blue) is a readymade stain reported to be used for capsule staining of Bacillus anthracis
  • Application
    For microbiological / pathological laboratories, hospitals, research settings and educational institutes. These stains augment tissue morphology, demonstrate important cellular components, aid in the evaluation of diseases, and identifies pathogenic organisms.
  • Classification
    Capsule Stains
  • Composition
    Methylene blue 0.300 gm 95% ethyl alcohol 30.000 ml Potassium hydroxide 0.010 gm Distilled water 100.000 ml
  • Appearance
    Deep violet coloured solution
  • Clarity
    Clear without any particles.
  • Microscopic Examination
    Capsule staining is carried out using M'Fadyean stain and staining characteristic is observed under microscope using oil immersion lens.
  • Results
    Bacillus anthracis ATCC 14578 : Pink capsule seen surrounding the deep bluish bacilli. Bacillus cereus ATCC 10876 : Deep bluish bacilli seen.
  • Storage
    Store below 30°C in tightly closed container and away from bright light.
  • Shelf Life
    Use before expiry date on label.
  • Properties
    Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.
  • Conjugation
    Blue Substrate
  • Gene target
  • Short name
    M’Fadyean Stain (Polychrome Methylene )
  • Label
    Blue
  • Alternative name
    M’Fadyean staining (Polychrome Methylene Blue)
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