-
Synonyms
Lyn B
-
Alternative_names
Lyn B
-
Description
A key enzyme involved in the regulation of cell activation
-
Recombinant
Yes
-
Source
Baculovirus (Sf9 insect cells)
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Activity Specifications test method
180 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
-
Molecular Weight
85.0 kDa
-
Storage Temp
-80°C
-
Shipping
dry ice
-
Shelf Life
12 months
-
Concentration
5µg/50 µl
-
Appearance
Liquid
-
Physical form description
Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
-
Background Information
Lyn is a 56 kd tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck and the v-yes protein as well as to the gene products of v-fgr and v-src. Northern hybridization analysis showed that a 3.2-kilobase Lyn mRNA was expressed in a variety of tissues of the human fetus (1). Lyn is expressed preferentially in B cells and can be coimmunoprecipitated with IgM suggesting that Lyn is physically associated with membrane-bound IgM, and participates in antigen-mediated signal transduction (2). Crosslinking of membrane-bound IgM with antibody induces rapid increase in activities of Lyn and Lyn-associated phosphatidylinositol 3-kinase (3). Crosslinking of B-cell antigen receptor also induces association of Lyn with an 85-kDa noncatalytic subunit of phosphatidylinositol 3-kinase. Thus, Lyn is functionally associated with membrane-bound IgM and participates in B-cell antigen receptor-mediated signaling.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
