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Available ordering format
Lyophilized
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Immunogen
Purified Rabbit IgG, whole molecule
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Raised in
Llama
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified llama IgG
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How to reconstitute
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
This antibody is suitable for all immunoassay applications
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Connected products
Other DyLight® anti-Rabbit secondary antibodies
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Recommended dilutions for use
needs to be determined by end user
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
reacts with: heavy (gamma) chains chains on rabbit IgG light chains on all on all rabbit immunoglobulins
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Possible reactivity
to be determined
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No reactivity
Based on IEP, no reactivity is observed to:Ë– non-immunoglobulin rabbit serum proteins
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Supplementary information
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References
to be added when available
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Scientific context
Llama anti-Rabbit IgG (H&L), DyLight 488 Conjugate is a secondary antibody which binds to heavy (gamma) chains chains on rabbit IgG light chains on all rabbit immunoglobulins.DyLight® 488 Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, 0.05 % (w/v) of Sodium azide as preservative.Based on IEP, this antibody reacts with: heavy (gamma) chains chains on rabbit IgG light chains on all on all rabbit immunoglobulins Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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About
Anti llama or alpaca camelloid antibodies to detect the antibodies of the domesticated South American camelid, widely used as production animal with phage display of recombinant llama antibodies.