Lipoamide Dehydrogenase Antibody

• Catalog number
  R30535
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Lipoamide Dehydrogenase
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Lipoamide Dehydrogenase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Lipoamide Dehydrogenase antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P09622
• Purity
  Antigen affinity
• Description
  DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex(BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.
• Immunogen
  An amino acid sequence from the C-terminus of human Lipoamide Dehydrogenase (EAFREANLAASFGKSINF) was used as the immunogen for this Lipoamide Dehydrogenase antibody.
• Storage
  After reconstitution, the Lipoamide Dehydrogenase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Lipoamide   Dehydrogenase  
• Gene symbol
  PDHB, PDHA2, OGDH
• Short name
  Anti-Lipoamide Dehydrogenase
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Lipoamide Dehydrogenase
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:8808
• Gene
  PDHB
• Long gene name
  pyruvate dehydrogenase E1 subunit beta
• Synonyms gene name
  • pyruvate dehydrogenase (lipoamide) beta
• Synonyms
  • PDHE1B
• Locus
  3p14.3
• Discovery year
  1989-06-30
• Entrez gene record
  5162
• Pubmed identfication
  • 17923481
• Classification
  • Pyruvate dehydrogenase complex
• VEGA ID
  OTTHUMG00000159157

Gene info

• Identity
  HGNC:8807
• Gene
  PDHA2
• Long gene name
  pyruvate dehydrogenase E1 subunit alpha 2
• Synonyms gene
  • PDHAL
• Synonyms gene name
  • pyruvate dehydrogenase (lipoamide) alpha 2
  • pyruvate dehydrogenase alpha 2
• Locus
  4q22.3
• Discovery year
  1990-07-11
• Entrez gene record
  5161
• VEGA ID
  OTTHUMG00000130990

Gene info

• Identity
  HGNC:8124
• Gene
  OGDH
• Long gene name
  oxoglutarate dehydrogenase
• Synonyms gene name
  • oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Synonyms
  • E1k
  • OGDC-E1
  • OGDH2
  • KGD1
• GenBank acession
  • D10523
• Locus
  7p13
• Discovery year
  1989-06-30
• Entrez gene record
  4967
• Pubmed identfication
  • 8020988
  • 1542694
  • 24506869
• Classification
  • Oxoglutarate dehydrogenase family
• VEGA ID
  OTTHUMG00000155304

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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