Phospholipase C Activity Assay Kit (Colorimetric)

• Catalog number
  K2016-100
• Price
  Please ask
• Size
  100 assays

• Description
  to determine the PLC activity
• Summary
  BioVision’s Phospholipase C Activity Assay Kit is a direct assay to determine the PLC activity in various biological samples. The assay uses a specific PLC chromogenic substrate to detect PLC activity and the generated final product can be measured colorimetrically at OD 405 nm. The OD signal is proportional to the PLC activity. The assay is rapid, sensitive and is a convenient tool for detecting PLC activity. It can detect as low as 0.1 mU PLC activity under the assay conditions.
• Detection Method
  Absorbance, OD 405 nm
• Species Reactivity
  All
• Applications
  Detection of PLC activity in various samples
• Sample Type
  Organs and tissues such as kidney, brain and muscle etc.
• Features Benefits
  A direct assay to determine the PLC activity in various biological samples., A convenient tool for detecting PLC activity., Sensitive, Rapid
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Phospholipase C (PLC) belongs to a family of membrane-associated enzyme that specifically cleaves the phosphoester linkage before the phosphate group in phospholipids. PLC catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG), which are second messengers critical for downstream signal pathways to control diverse cellular processes. PLC family consists of 13 isozymes split between six subfamilies. Members of the PLC family are involved in intra and inter cellular signal transduction pathways that are important for cell proliferation and differentiation. PLC isozymes have been also associated with several diseases such as cancer, inflammation, cardiovascular diseases and neuropathic pain. Therefore, investigation of PLC enzymes and their inhibition may lead to innovative therapeutic strategies. BioVision’s Phospholipase C Activity Assay Kit is a direct assay to determine the PLC activity in various biological samples. The assay uses a specific PLC chromogenic substrate to detect PLC activity and the generated final product can be measured colorimetrically at OD 405 nm. The OD signal is proportional to the PLC activity. The assay is rapid, sensitive and is a convenient tool for detecting PLC activity. It can detect as low as 0.1 mU PLC activity under the assay conditions.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Phospholipase   Activity   Kit  
• Short name
  Phospholipase C Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Phospholipase C Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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