Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC

• Catalog number
  6K01-T050
• Price
  Please ask
• Size
  50 tests

• Properties
  This Exbio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exbio FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Lineage   Cocktail   CD3   CD14   CD16   CD19   CD20   CD56  
• Gene symbol
  CD19, CD14, NCAM1, MS4A1, FCGR3A, FCGR3B
• Short name
  Lineage Cocktail (CD3 / CD14 / CD16 / CD19 / CD20 / CD56) FITC
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  Lineage Cocktail (CD3 / CD14 molecule / CD16 / CD19 molecule / CD20 / CD56) fluorecein
• Alternative technique
  fluorescine
• Alternative to gene target
  CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
• Virus
  lineage

Gene info

• Identity
  HGNC:1633
• Gene
  CD19
• Long gene name
  CD19 molecule
• Synonyms gene name
  • CD19 antigen
• Locus
  16p11.2
• Discovery year
  1991-06-04
• Entrez gene record
  930
• Classification
  • CD molecules
  • Immunoglobulin like domain containing
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000097049
• Locus Specific Databases
  • CD19base: Mutation registry for CD19 deficiency
  • LRG_35

Gene info

• Identity
  HGNC:1628
• Gene
  CD14
• Long gene name
  CD14 molecule
• Synonyms gene name
  • CD14 antigen
• Locus
  5q31.3
• Discovery year
  1988-05-31
• Entrez gene record
  929
• Pubmed identfication
  • 2472171
  • 2462937
• RefSeq identity
  • NM_000591
• Classification
  • CD molecules
  • Scavenger receptors
• VEGA ID
  OTTHUMG00000129507

Gene info

• Identity
  HGNC:7656
• Gene
  NCAM1
• Long gene name
  neural cell adhesion molecule 1
• Synonyms
  • NCAM
  • CD56
• Locus
  11q23.2
• Discovery year
  2001-06-22
• Entrez gene record
  4684
• RefSeq identity
  • NM_000615
• Classification
  • Fibronectin type III domain containing
  • CD molecules
  • Ig-like cell adhesion molecule family
  • I-set domain containing
• VEGA ID
  OTTHUMG00000167196

Gene info

• Identity
  HGNC:7315
• Gene
  MS4A1
• Long gene name
  membrane spanning 4-domains A1
• Synonyms gene
  • CD20
• Synonyms gene name
  • membrane-spanning 4-domains, subfamily A, member 1
• Synonyms
  • B1
  • Bp35
  • FMC7
• GenBank acession
  • M27394
• Locus
  11q12.2
• Discovery year
  1989-05-23
• Entrez gene record
  931
• Pubmed identfication
  • 2448768
• Classification
  • Membrane spanning 4-domains
  • CD molecules
• VEGA ID
  OTTHUMG00000167614
• Locus Specific Databases
  • LRG_140

Gene info

• Identity
  HGNC:3619
• Gene
  FCGR3A
• Long gene name
  Fc gamma receptor IIIa
• Synonyms gene
  • FCGR3
  • FCG3
• Synonyms gene name
  • Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
  • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
  • Fc fragment of IgG receptor IIIa
• Synonyms
  • CD16
  • CD16a
  • FcgammaRIIIa
  • FcGRIIIA
• GenBank acession
  • BC036723
• Locus
  1q23.3
• Discovery year
  1988-11-30
• Entrez gene record
  2214
• Pubmed identfication
  • 2139735
  • 11835346
  • 19309690
• RefSeq identity
  • NM_000569
• Classification
  • Immunoglobulin like domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000034466
• Locus Specific Databases
  • FCGR3Abase: Mutation registry for Natural killer cell deficiency
  • LRG_60

Gene info

• Identity
  HGNC:3620
• Gene
  FCGR3B
• Long gene name
  Fc gamma receptor IIIb
• Synonyms gene
  • FCGR3
  • FCG3
• Synonyms gene name
  • Fc fragment of IgG, low affinity IIIb, receptor for (CD16)
  • Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
  • Fc fragment of IgG receptor IIIb
• Synonyms
  • CD16
  • CD16b
  • FcgammaRIIIb
  • FcRIIIb
• GenBank acession
  • J04162
• Locus
  1q23.3
• Discovery year
  1991-08-21
• Entrez gene record
  2215
• Pubmed identfication
  • 2139735
  • 11034564
  • 17529978
• RefSeq identity
  • NM_000570
• Classification
  • CD molecules
  • Immunoglobulin like domain containing
• VEGA ID
  OTTHUMG00000074099
• Locus Specific Databases
  • LRG_1238

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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