Lin-28 Antibody, ALEXA FLUOR 647
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Catalog number
bs-6446R-A647
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Price
Please ask
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Size
100ul
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Long name
Lin-28 Polyclonal Antibody, ALEXA FLUOR 647 Conjugated
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Also known as
Anti-Lin-28 PAb ALEXA FLUOR 647
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 647
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Lin-28
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Specificity
This is a highly specific antibody against Lin-28.
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Modification
Unmodified
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Modification Site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
70-120/250
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Subcellular location
Cytoplasm, Nucleus
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Lin-28B
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Gene ID Number
389421, 79727
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Swiss Prot
Q6ZN17,Q9H9Z2
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
650nm/665nm
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Synonyms
Lin28B; CSDD 2; CSDD2; FLJ16517; Lin 28 homolog B C. elegans; Lin 28.2; Lin-28B; LIN28B; LN28B_HUMAN; Protein lin-28 homolog B; Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1.
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-Lin-28 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor, ALEXA FLUOR 647
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French translation
anticorps
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Gene target
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Gene symbol
LIN28A, LIN28B, LIN28AP2
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Short name
Anti-Lin-28
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 647
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Alternative name
Lin-28 Polyclonal Antibody, ALEXA FLUOR 647
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
lin-28 homolog A
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Synonyms gene
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Synonyms gene name
- lin-28 homolog (C. elegans)
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Synonyms
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GenBank acession
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Locus
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Discovery year
2003-07-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
lin-28 homolog B
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Synonyms gene name
- lin-28 homolog B (C. elegans)
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Synonyms
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GenBank acession
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Locus
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Discovery year
2005-08-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
LIN28A pseudogene 2
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Synonyms gene name
- lin-28 homolog A (C. elegans) pseudogene 2
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Locus
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Discovery year
2010-04-06
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Entrez gene record
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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