LIMK1 Antibody

• Catalog number
  bs-2775R
• Price
  Please ask
• Size
  0.1ml

• Long name
  LIMK1 Primary Polyclonal Antibody
• Also known as
  LIMK1 PAb
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  LIMK1
• Specificity
  This is a highly specific antibody against LIMK1.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Polyclonal antibody
• Clone number
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  465-512/647
• Subcellular location
  Cytoplasm, Nucleus
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human LIMK1
• Gene ID number
  3984
• Swiss Prot
  P53667
• Tested Applications
  WB, IHC-P, IF(IHC-P)
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
• Cross reactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  LIMK; LIMK-1; LIM domain kinase 1; LIMK1
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  LIMK1  
• Gene symbol
  LIMK1
• Short name
  Anti-LIMK1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  Immunoglobulin G (IgG)
• Alternative name
  LIMK1 Polyclonal Antibody
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6613
• Gene
  LIMK1
• Long gene name
  LIM domain kinase 1
• Synonyms
  • LIMK
• GenBank acession
  • D26309
• Locus
  7q11.23
• Discovery year
  1996-03-14
• Entrez gene record
  3984
• Pubmed identfication
  • 8673124
  • 8812460
• RefSeq identity
  • NM_002314
• Classification
  • LIM domain containing
  • PDZ domain containing
  • LIMK/TESK kinase family
• VEGA ID
  OTTHUMG00000023448

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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