LIM kinase 1 + 2 Antibody, ALEXA FLUOR 488

• Catalog number
  bs-11871R-A488
• Price
  Please ask
• Size
  100ul

• Long name
  LIM kinase 1 + 2 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
• Also known as
  Anti-LIM kinase 1 + 2 PAb ALEXA FLUOR 488
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 488
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  LIM kinase 1 + 2
• Specificity
  This is a highly specific antibody against LIM kinase 1 + 2.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human LIM kinase 1 + 2
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  LIMK 1 and 2 likely regulate aspects of the cytoskeleton, through control of the organization of actin filaments. They can phosphorylate an actin-binding protein, cofilin which binds to actin monomers and polymers and promotes the disassembly of actin filament.The phosphorylation of cofilin via LIMK inactivates this potential. LIMK1 is highly active in the brain and spinal chord, where it is believed to be involved in the development of nerve cells whilst LIMK2 is ubiquitously expressed in many adult tissues. LIMK1 may play an important role in areas of the brain that are responsible for processing visual-spatial information (visuospatial constructive cognition). These parts of the brain are important for visualizing an object as a set of parts and performing tasks such as writing, drawing, constructing models, and assembling puzzles. LIMK1 is specifically stimulated by Rac, one of the Rho family proteins, while LIMK2 activity is activated under the control of other Rho family members, Rho and Cdc42, suggesting that two distinct pathways exist in the Rho family driven actin cytoskeleton dynamics.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  499nm/519nm
• Synonyms
  LIM kinase 1+2; LIMK1+LIMK2; LIM domain kinase 1; LIM domain kinase 2; LIMK1; LIMK2;LIMK1_HUMAN; LIMK2_HUMAN.
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-LIM kinase 1 + 2 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor
• French translation
  anticorps

• Gene target
  LIM   kinase  
• Gene symbol
  PDLIM5, LHX1
• Short name
  Anti-LIM kinase 1 + 2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 488
• Alternative name
  LIM kinase 1 + 2 Polyclonal Antibody, ALEXA FLUOR 488
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:17468
• Gene
  PDLIM5
• Long gene name
  PDZ and LIM domain 5
• Synonyms
  • LIM
  • Enh
• GenBank acession
  • AF061258
• Locus
  4q22.3
• Discovery year
  2005-01-12
• Entrez gene record
  10611
• Pubmed identfication
  • 15346770
• Classification
  • PDZ domain containing
  • LIM domain containing
• VEGA ID
  OTTHUMG00000130973

Gene info

• Identity
  HGNC:6593
• Gene
  LHX1
• Long gene name
  LIM homeobox 1
• Synonyms
  • LIM-1
  • LIM1
• GenBank acession
  • U14755
• Locus
  17q12
• Discovery year
  1996-10-02
• Entrez gene record
  3975
• Pubmed identfication
  • 9212161
• RefSeq identity
  • NM_005568
• Classification
  • LIM class homeoboxes
• VEGA ID
  OTTHUMG00000188456

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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