Recombinant Mouse AKT3 Protein (aa 106-479, His & GST tag) 50µg

• Catalog number
  PKSM040385-50µg
• Price
  Please ask
• Size
  50µg

• Activity
  NA
• Synonym
  AI851531;D930002M15Rik;Nmf350
• Sequence
  Ala106-Glu479
• Fusion tag
  N-His & GST
• Accession
  Q9WUA6-1
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 90 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  71 kDa
• AP Mol Mass
  65 kDa
• Formulation
  Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol
• Background
  v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  AKT3   Protein   106-479   His   GST   tag   50µg  
• Gene symbol
  AKT3-IT1, MGST3, PRELID1, AKT3, OR8C1P
• Short name
  Recombinant Mouse AKT3 Protein (aa 106-479, His & GST tag) 50µg
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse AKT3 Protein (aa 106-479, histidine & GST detection labelled) 50µg
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:41304
• Gene
  AKT3-IT1
• Long gene name
  AKT3 intronic transcript 1
• Synonyms gene name
  • AKT3 intronic transcript 1 (non-protein coding)
• Locus
  1q44
• Discovery year
  2011-05-20
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000039993

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:30255
• Gene
  PRELID1
• Long gene name
  PRELI domain containing 1
• Synonyms
  • CGI-106
  • PX19
  • PRELI
• Synonyms name
  • protein of relevant evolutionary and lymphoid interest
  • px19-like protein
• GenBank acession
  • BC013748
• Locus
  5q35.3
• Discovery year
  2007-02-20
• Entrez gene record
  27166
• Pubmed identfication
  • 10784606
  • 14640972
• RefSeq identity
  • NM_013237
• Classification
  • PRELI domain containing
• VEGA ID
  OTTHUMG00000130847

Gene info

• Identity
  HGNC:393
• Gene
  AKT3
• Long gene name
  AKT serine/threonine kinase 3
• Synonyms gene name
  • v-akt murine thymoma viral oncogene homolog 3
• Synonyms
  • PKBG
  • RAC-gamma
  • PRKBG
• Synonyms name
  • protein kinase B, gamma
• GenBank acession
  • AF124141
• Locus
  1q43-q44
• Discovery year
  1999-11-16
• Entrez gene record
  10000
• Pubmed identfication
  • 10092583
  • 10208883
• RefSeq identity
  • NM_181690
• Classification
  • Pleckstrin homology domain containing
  • AKT kinases
• VEGA ID
  OTTHUMG00000039994
• Locus Specific Databases
  • LRG_1396

Gene info

• Identity
  HGNC:8478
• Gene
  OR8C1P
• Long gene name
  olfactory receptor family 8 subfamily C member 1 pseudogene
• Synonyms gene
  • OR8C3P
  • OR8C4P
• Synonyms gene name
  • olfactory receptor, family 8, subfamily C, member 1 pseudogene
• Synonyms
  • OR11-175
  • OR912-45
  • OR912-106
• GenBank acession
  • AF065872
• Locus
  11q24.2
• Discovery year
  1998-11-09
• Entrez gene record
  8580
• Pubmed identfication
  • 9787077
• Classification
  • Olfactory receptors, family 8
• VEGA ID
  OTTHUMG00000154390

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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