Lenti-CMV-hTERT-GFP-2A-Puro

Ordering information

The catalog number at Gentaur for this research reagent is 171-LV623. It is a frequent ordered item.
Delivery

The Lenti-CMV-hTERT-GFP-2A-Puro can be delivered to your laboratory the next week after your order if you order before Friday 15:00.
Users reference

Contact us for laboratory references of researchers that recently used this product in Europe or the United States.
Description

Lenti kit or vector with CMV or EF promotors for producing Lentivirus (lenti-, Latin for "slow") or lentiviral particles in 293t cells. Lentiviruses are characterized by a long incubation period and often Puro selected. Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells. They are one of the most efficient methods of a gene delivery vector. The are packaged in HEK 293T cells to get the highest possible lentiviral titers of 108 IFU/ml. The Luc reporter is the expression of the firefly luciferase gene. Puro stands for puromicin selection.

Gene target

CMV-hTERT-GFP-2A-Puro
Gene symbol

IRX5, IGHD2OR15-2A, EIF2A, CXCL2, RNVU1-2A, PLPP1, CHD3, TRAPPC2B, TRAPPC2, MYH2
Short name

-CMV-hTERT-GFP-2A-Puro
Technique

lenti, Lentivectors and viral particles
Label

gfp
Alternative name

lentiviral-cytomegalovirus-hTERT-Green Fluorescent-2A-Puro
Virus

cmv, lenti

Identity

HGNC:1918
Gene

CHD3
Long gene name

chromodomain helicase DNA binding protein 3
Synonyms
- Mi-2a
- ZFH
- Mi2-ALPHA
GenBank acession
- U08379
Locus

17p13.1
Discovery year

1998-03-20
Entrez gene record

1107
Pubmed identfication
- 9326634
- 7560064
RefSeq identity
- NM_001005273
Classification
- Small nucleolar RNA protein coding host genes
- NuRD complex subunits
- PHD finger proteins
VEGA ID

OTTHUMG00000150427

Identity

HGNC:10710
Gene

TRAPPC2B
Long gene name

trafficking protein particle complex subunit 2B
Synonyms gene
- SEDLP
- TRAPPC2P1
Synonyms gene name
- spondyloepiphyseal dysplasia, late, pseudogene
- trafficking protein particle complex 2 pseudogene 1
- trafficking protein particle complex 2B
Synonyms
- SEDLP1
- MIP-2A
Locus

19q13.43
Discovery year

2000-01-20
Entrez gene record

10597
Pubmed identfication
- 11134351
- 24098805
RefSeq identity
- NM_001355204
VEGA ID

OTTHUMG00000183279

Identity

HGNC:23068
Gene

TRAPPC2
Long gene name

trafficking protein particle complex subunit 2
Synonyms gene
- SEDL
Synonyms gene name
- spondyloepiphyseal dysplasia, late
- trafficking protein particle complex 2
Synonyms
- TRS20
- SEDT
- MIP-2A
- ZNF547L
- hYP38334
GenBank acession
- AF157061
Locus

Xp22.2
Discovery year

2005-01-26
Entrez gene record

6399
Pubmed identfication
- 14597397
RefSeq identity
- NM_014563
Classification
- Trafficking protein particle complex subunits
VEGA ID

OTTHUMG00000021157

Identity

HGNC:7572
Gene

MYH2
Long gene name

myosin heavy chain 2
Synonyms gene
- IBM3
Synonyms gene name
- myosin, heavy polypeptide 2, skeletal muscle, adult
- inclusion body myopathy 3, autosomal dominant
Synonyms
- MYH2A
- MYHSA2
- MyHC-IIa
- MYHas8
- MyHC-2A
Locus

17p13.1
Discovery year

2001-06-22
Entrez gene record

4620
Pubmed identfication
- 7545970
- 11889243
RefSeq identity
- NM_017534
Classification
- Myosin heavy chains, class II
VEGA ID

OTTHUMG00000130363

Name

Artificial Gene Fusion
Concept

Scope note: The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
Tree numbers
- E05.393.220.250
Qualifiers

drug effects, methods, radiation effects