LB Growth Medium w/o Sodium Chloride

Description

LB Growth Medium w/o Sodium Chloride is employed in the protein espression system where sodium chloride is used to induce protein. To prepre the media d issolve the powder in d istilled water, heat to boiling if it is necessary, then d ispense as desired and sterilize by autoclaving at 121°C for 15 minutes.
Other product
Application

Molecular Biology Growth Media for Escherichia coli
Quantity required by litre

15 g
Appearance of powder

Light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity

Light yellow coloured, clear solution without any precipitate.
Cultural Response

Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
Organisms ATCC Growth

Escherichia coli: good-luxuriant
Composition g l

Tryptone 10.00 Yeast extract 5.00
Storage

Store below 30°C and the prepared medium at 2 - 8°C.
Additional description

There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
Group

media

Name

In Vitro Techniques
Concept

Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
Tree numbers
- E05.481
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data