Klotho Recombinant Protein

• Catalog number
  40-195
• Price
  Please ask
• Size
  0.005 mg, 0.02 mg

• About recombinant protein
  Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. The full length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively, The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid. This circulating protein arises from the action of an as yet unidentified protease which cleaves transmembrane Klotho just above and/or within the plasma membrane. Klotho has been shown to play a key role in the signaling cascade of fibroblast growth factor-23 (FGF-23), a bone derived hormone that acts in the kidney to inhibit phosphate reabsorption and vitamin D biosynthesis. Klotho promotes FGF-23 signaling through binding to FGFRI (IIIc) which converts this canonical FGF receptor into a specific receptor for FGF-23. In the absence of Klotho the function of FGF-23 is literally abolished. Recombinant human Klotho is a 65-70 kDa glycoprotein containing 516 amino acid residues.
• Other name of the recombinant
  Klotho
• Recombinant in
  CHO cells
• SEQUENCE
  EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH
• Activity
  Biological activity was determined by the dose - dependent stimulation of the proliferation of murine NIH - 3T3 cells. Recombinant human Klotho is effective in a concentration range of 0.5 - 2.0 μg/mL.
• Keep at
  The lyophilized Klotho recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Klotho is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Klotho recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  24497614
• Accession nr
  NP_004786.2
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  9365
• Gene name
  klotho
• Source
  Homo sapiens
• Uniprot
  Q9UEF7
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Klotho   Protein  
• Gene symbol
  KL
• Short name
  Klotho Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Klotho Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:6344
• Gene
  KL
• Long gene name
  klotho
• Synonyms
  • KLA
• Synonyms name
  • alpha-klotho
• GenBank acession
  • AB005142
• Locus
  13q13.1
• Discovery year
  1999-03-24
• Entrez gene record
  9365
• Pubmed identfication
  • 9464267
• Classification
  • Glycoside hydrolase family 1
• VEGA ID
  OTTHUMG00000017408
• Locus Specific Databases
  • The Globin Gene Server

Gene info

• Identity
  HGNC:15583
• Gene
  LCTL
• Long gene name
  lactase like
• Synonyms
  • KLPH
  • FLJ33279
  • KLG
• Synonyms name
  • klotho gamma
  • KL lactase phlorizin hydrolase
  • gammaklotho
• GenBank acession
  • AY358729
• Locus
  15q22.31
• Discovery year
  2005-07-26
• Entrez gene record
  197021
• Pubmed identfication
  • 12084582
• RefSeq identity
  • NM_207338
• Classification
  • Glycoside hydrolase family 1
• VEGA ID
  OTTHUMG00000133207

Gene info

• Identity
  HGNC:372
• Gene
  AKAP2
• Long gene name
  A-kinase anchoring protein 2
• Synonyms gene
  • PRKA2
• Synonyms gene name
  • A kinase (PRKA) anchor protein 2
• Synonyms
  • AKAP-KL
  • KIAA0920
  • DKFZp564L0716
  • MISP2
• Synonyms name
  • protein kinase A2
• GenBank acession
  • AB023137
• Locus
  9q31.3
• Discovery year
  1999-09-16
• Pubmed identfication
  • 10231032
• Classification
  • A-kinase anchoring proteins
• VEGA ID
  OTTHUMG00000156811

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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