KLK3 (Kallikrein-3), human recombinant
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Catalog number
4727-100
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Price
Please ask
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Size
100 ug
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Synonyms
APS, KLK2A1, PSA, Hk3
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Alternative_names
APS, KLK2A1, PSA, Hk3, P-30 antigen, Semenogelase
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Description
A glycoprotein enzyme elevated in prostate cancer
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Recombinant
Yes
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Source
Human
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >100 pmoles/min/ug
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Results
>100 pmoles/min/ug
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Molecular Weight
35 kDa
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Storage Temp
-70°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Lyophilized from sterile PBS, pH 7.4. (Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.)
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Reconstitution Instructions
Detailed reconstitution instructions are sent along with the product.
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Background Information
Prostate-specific antigen, also known as Gamma-seminoprotein, Kallikrein-3, kallikrein-related peptidase 3, Semenogelase, KLK3 and PSA, is a secreted protein which belongs to thepeptidase S1 family and Kallikrein subfamily. KLK3/Kallikrein 3 contains one peptidase S1 domain. KLK3/Kallikrein 3 is a glycoprotein produced almost exclusively by the prostate gland. KLK3/Kallikrein 3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.It is also believed to be instrumental in dissolving the cervical mucous, allowing the entry of sperm. Human KLK3/Kallikrein 3 and human KLK2 are closely related products of the human kallikrein genes KLK3 and KLK2, respectively. Both KLK3/Kallikrein 3 and human kallikrein 2 are produced and secreted in the prostate and have important applications in the diagnosis of prostate cancer. Understanding the mechanism by which genetic variation in KLK3/Kallikrein 3 affects prostate cancer risk has important implications for study of the biological role of KLK3/Kallikrein 3 in prostate tumorigenesis.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
KLKB1, KLK3
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Short name
KLK3 (Kallikrein-3), recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
KLK3 (Kallikrein-3), H. sapiens Rec.
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
kallikrein B1
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Synonyms gene
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Synonyms gene name
- kallikrein B, plasma (Fletcher factor) 1
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1999-04-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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