Kappa light chain (HP6053) Monoclonal Antibody
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Catalog number
bsm-51106M
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Price
Please ask
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Size
0.1ml
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Long name
Kappa light chain (HP6053) Monoclonal Antibody
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Also known as
Kappa light chain (HP6053) Monoclonal Antibody
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Category
Primary Antibodies
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Conjugation
Unconjugated
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Target Antigen
Kappa light chain
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Specificity
This is a highly specific antibody against Kappa light chain.
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Modification s
None
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Modification site s
Unmodified antibody
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Clonality
Monoclonal
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Clone number
HP6053
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Source
This Kappa light chain monoclonal antibody is generated from mouse immunized with Kappa light chain recombinant protein.
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Swiss Prot
P01834
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Tested Applications
IHC-P
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Recommended dilutions
IHC-P(1:10~50)
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Cross reactivity
Human
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigen
Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains designated as kappa and lambda (1). Light chain types are based on differences in the amino acid sequence in the constant region of the light chain. If a cell is unsuccessful in rearranging both of its kappa light chain genes, it then attempts to make a lambda light chain. If a cell successfully rearranges a lambda light chain gene, it will be a B cell that makes an immunoglobulin with a lambda light chain (2).
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Purification method
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Short name
Anti-Kappa light chain
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Technique
Antibody, light, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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Host
Mouse (Mus musculus)
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Isotype
Immunoglobulin G (IgG)1 κ
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Alternative name
Kappa light chain (HP6053) Monoclonal Antibody
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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