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Synonyms
K63-linked Tri-Ubiquitin
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Description
Substrate for defined length polyubiquitination
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Purity by SDS PAGE
≥95%
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Assay
Western Blot
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Molecular Weight
25.686 kDa (Band migrates faster on gels)
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Storage Temp
-80°C
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Shipping
Dry ice
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Shelf Life
12 months
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Concentration
2.5 mg/ml
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Appearance
Liquid
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Physical form description
2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.
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Background Information
Poly-ubiquitination of target proteins through K63 has recently become the focus of intense study. The topology of this linkage type is quite different from polyubiquitin linked through lysine 48. Modification of proteins by K63-linked polyubiquitination has been implicated in, among other cellular processes, the regulation of the DNA damage response, endosomal sorting, autophagy of misfolded/aggregated proteins, and neurodegeneration. These tri-ubiquitin chains are generated from the enzymatic linkage of wild-type ubiquitin through lysine 63. The most distal ubiquitin contains an arginine substitution for a lysine at position 63, limiting chain length.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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