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Synonyms
K11-linked Di-Ubiquitin
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Description
Substrate for defined length polyubiquitination
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Purity by SDS PAGE
≥95%
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Assay
Western Blot
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Molecular Weight
17.111 kDa (Band migrates faster on gels)
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Storage Temp
-80°C
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Shipping
Dry ice
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Shelf Life
12 months
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Concentration
2.5 mg/ml
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Appearance
Liquid
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Physical form description
2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.
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Background Information
Increasingly, researchers are focusing on the role poly-ubiquitin chains linked through K11. This post-translational modification has been linked to the ERAD cycle as a signal, similar to K48 linkage, for proteasomal degradation. More recently, K11 linkage appears to play an important role in cell cycle signaling, as it is associated with the anaphase promoting complex (APC) of ubiquitination machinery. These di-ubiquitin chains are generated from the enzymatic linkage (UBE2S) of wild-type ubiquitin through lysine 11 and purified to >95% homogeneity by ion exchange chromatography.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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