JNK Activity Screening Kit, KinaseSTAR

Summary

• Detection method- Western blot analysis with anti-Phospho-cJun (Ser 73) Specific Antibody at 1:1000 dilutions • Species reactivity- Mammalian • Applications- The kit can be used to screen JNK activity relatively quickly for large numbers of samples.
Sample Type

Cell and tissue lysates
Features Benefits

• Simple procedure • The kit is suitable for both research and drug discovery • The assay utilizes a N-terminal c-Jun fusion protein bead to selectively “pull down” JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody.
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity screening kit utilizes an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively “pull down” JNK from cell lysates. After wash to remove nonspecifically bound proteins, the kinase reaction is then carried out in the presence of cold ATP. c-Jun phosphorylation is then measured by Western blot analysis using a phospho-c-Jun specific antibody.
Data Sheet Link

Gene target

JNK Activity Screening Kit KinaseSTAR
Gene symbol

MAPK8
Short name

JNK Activity Screening Kit, KinaseSTAR
Alternative name

JNK Activity detection reagent, KinaseSTAR
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832