Iscove's Modified DMEM (IMDM) w/ L-glutamine and 25 mM HEPES No sodium bicarbonate

  • Catalog number
    IMP04-50LT
  • Price
    Please ask
  • Size
    50 L
  • Availability
    Please contact us to check current availability
  • Long description
    Iscove's Modified DMEM (IMDM) w/ L-glutamine and 25 mM HEPES No sodium bicarbonate Medium
  • Product format
    Powder
  • Product category
    Animal Cell Culture Media
  • Product subcategory
    Iscove's Modified DMEM
  • Shipping method
    Ambient temperature
  • Storage method
    Store at 2˚ to 8˚C.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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