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Long name
Eph receptor B1(Y968) Antibody, ALEXA FLUOR 488 Conjugated
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Also known as
Anti-Eph receptor B1 Tyr968 PAb ALEXA FLUOR 488
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 488
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Eph receptor B1 Tyr968
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Specificity
This is a highly specific antibody against Eph receptor B1 Tyr968.
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Modification
Phosphorylation
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Modification Site
Tyr968
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Subcellular location
Extracellular
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Source
KLH conjugated synthetic phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr968
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Gene ID Number
2047
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
EphB1, previously known as Elk (eph like kinase), is a receptor tyrosine kinase of the highly tissue restricted family of eph proteins. EphB1 and other ephB family members are type 1 membrane spanning proteins, comprised of immunoglobulin, fibronectin type III, and cysteine rich subdomains in the ecto domain, and the single uninterrupted cytoplasmic tyrosine kinase domain upstream of a carboxyterminal sterile alpha motif (SAM) domain. EphB family proteins bind ephrins of the B class. EphB1 is expressed predominately in developing neural structures in embryos, and in vascular epithelium of kidney, and other tissues. Upon binding to alternatively oligomerized ephrin B1, EphB1 signals regulation of cell attachment and cell to cell assembly. Members of this protein family are implicated in neuronal and vascular cell targeting.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
499nm/519nm
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Synonyms
Eph receptor B1phospho Y968; EphB1phospho Y968Cek 6; Elkh; Eph tyrosine kinase 2; EPH2; EphB1; Ephrin receptor Eph B1 precursor; Ephrin type B receptor 1; HEK 6; NET; Neuronally expressed EPH related tyrosine kinase; Tyrosine protein kinase receptor EPH 2.
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Eph receptor B1 Tyr968 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor
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Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translation
anticorps