Inosine Fluorometric Assay Kit

Summary

• Detection method- Fluorescence (Ex/Em = 535/587 nm) • Species reactivity- Mammalian • Application- This assay has a detection limit of of approximately 100 pmol inosine/well.
Sample Type

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, etc.
Features Benefits

• Simple procedure; takes ~ less than 40 minutes • Fast and convenient • Sensitive assays for measuring Inosine levels in a variety of samples.
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia. In BioVision’s Inosine Assay Kit, inosine is converted to hypoxanthine, which will react with the substrate mix and PicoProbe™ to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.
Data Sheet Link
Test

Biovision supplies other types of Assays as 1.

Gene target

Inosine Fluorometric Kit
Short name

Inosine Fluorometric Assay Kit
Technique

Assay, assays
Host

Assay
Alternative name

Inosine fluorescence test reagent
Alternative technique

arrays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832