IMDM w/ L- Glutamine w/ 25mM Hepes - 500ml
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Catalog number
LM-I1090/500
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Price
Please ask
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Size
20X500ml
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Description
IMDM w/ L- Glutamine w/ 25mM Hepes - 500ml is excellent for rapidly proliferating high-density cell cultures.
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Shipping conditions
-20°C or dry ice
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Storage
+2/+8°C
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Shelflife
12 months
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State
Liquid
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Chemical description
Water-based mixture of inorganic and organic compounds
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Appearance
Physical state at 20 °C : Liquid ; Appearance: Fluid ; Colour : Red
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Density at 20 ºC
1040 kg/m³
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Relative density at 20 ºC
1.04
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PH
7.2 ± 0.3
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Osmolality
276 ±10% mOsm/kg
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Endotoxin
< 1 EU/ml
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Cell growth test
Medium tested for the ability to support SP2/0-Ag14 cell growth.
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Composition
NA
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Sterility
Sterile filtered
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Personal precautions
Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
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Uses
Usage biotechnologique. Uniquement pour usage professionnel.
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Ordering
To order IMDM w/ L- Glutamine w/ 25mM Hepes - 500ml, please use the Cat. Nr.LM-I1090/500 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for IMDM w/ L- Glutamine w/ 25mM Hepes - 500ml
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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