Leptin Recombinant Protein
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Catalog number
40-289
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Price
Please ask
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Size
0.2 mg, 1.0 mg
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About recombinant protein
Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant human Leptin is a 16.0 kDa protein containing 147 amino acid residues.
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Other name of the recombinant
OB, OBS, LEPD, OB, Leptin, Obese protein
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Recombinant in
E. coli
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SEQUENCE
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
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Activity
Human Leptin is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 μg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline - treated controls.
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Keep at
The lyophilized Leptin recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Leptin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Leptin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
4557715
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Accession nr
NP_000221.1
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
3952
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Gene name
leptin
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Source
Homo sapiens
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Uniprot
P41159
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Gene
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
LEP
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Short name
Leptin Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
Leptin Rec. Protein
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
leptin
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Synonyms gene
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Synonyms gene name
- leptin (murine obesity homolog)
- leptin (obesity homolog, mouse)
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Locus
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Discovery year
1993-01-26
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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