GM-CSF

• Catalog number
  E21-003
• Price
  Please ask
• Size
  10ug

• Shipping_Temperature
  Ambient
• Product_State
  Lyophilized
• Formulation_Description
  Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Mannitol, pH 7.2.
• Storage
  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Reconstitution
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Background
  Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
• Alternative Names
  Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
• Expression system
  E.coli
• Gene
  Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.

• Gene target
  GM-CSF  
• Gene symbol
  CSF2, PPP1R3A, CSF1, CSF3, IL3
• Short name
  GM-CSF
• Species
  Human
• Alternative name
  GM-CSF

Gene info

• Identity
  HGNC:2434
• Gene
  CSF2
• Long gene name
  colony stimulating factor 2
• Synonyms gene name
  • colony stimulating factor 2 (granulocyte-macrophage)
• Synonyms
  • GM-CSF
  • GMCSF
• Synonyms name
  • sargramostim
  • molgramostim
  • granulocyte-macrophage colony stimulating factor
• GenBank acession
  • M11734
• Locus
  5q31.1
• Discovery year
  2001-06-22
• Entrez gene record
  1437
• Pubmed identfication
  • 3898082
  • 2999978
• RefSeq identity
  • NM_000758
• VEGA ID
  OTTHUMG00000059637

Gene info

• Identity
  HGNC:9291
• Gene
  PPP1R3A
• Long gene name
  protein phosphatase 1 regulatory subunit 3A
• Synonyms gene
  • PPP1R3
• Synonyms gene name
  • protein phosphatase 1, regulatory (inhibitor) subunit 3A (glycogen and sarcoplasmic reticulum binding subunit, skeletal muscle)
  • protein phosphatase 1, regulatory (inhibitor) subunit 3A
  • protein phosphatase 1, regulatory subunit 3A
• Synonyms
  • GM
• Synonyms name
  • glycogen-associated regulatory subunit of protein phosphatase-1
  • protein phosphatase 1 regulatory subunit GM
• GenBank acession
  • AF024578
• Locus
  7q31.1
• Discovery year
  1993-01-22
• Entrez gene record
  5506
• Pubmed identfication
  • 7926294
• RefSeq identity
  • NM_002711
• Classification
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000156944

Gene info

• Identity
  HGNC:2432
• Gene
  CSF1
• Long gene name
  colony stimulating factor 1
• Synonyms gene name
  • colony stimulating factor 1 (macrophage)
• Synonyms
  • M-CSF
  • MCSF
  • MGC31930
• Synonyms name
  • macrophage colony stimulating factor 1
• GenBank acession
  • BC021117
• Locus
  1p13.3
• Discovery year
  1986-01-01
• Entrez gene record
  1435
• Pubmed identfication
  • 1540160
• RefSeq identity
  • NM_000757
• VEGA ID
  OTTHUMG00000011646

Gene info

• Identity
  HGNC:2438
• Gene
  CSF3
• Long gene name
  colony stimulating factor 3
• Synonyms gene
  • GCSF
  • G-CSF
  • C17orf33
• Synonyms gene name
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
• Synonyms
  • MGC45931
• Synonyms name
  • granulocyte colony stimulating factor
  • pluripoietin
  • filgrastim
  • lenograstim
• Locus
  17q21.1
• Discovery year
  2001-06-22
• Entrez gene record
  1440
• Pubmed identfication
  • 3499671
  • 3501046
• RefSeq identity
  • NM_172220
• Classification
  • Receptor ligands
• VEGA ID
  OTTHUMG00000133247

Gene info

• Identity
  HGNC:6011
• Gene
  IL3
• Long gene name
  interleukin 3
• Synonyms gene name
  • interleukin 3 (colony-stimulating factor, multiple)
• Synonyms
  • IL-3
  • MULTI-CSF
  • MCGF
  • MGC79398
  • MGC79399
• Synonyms name
  • multilineage-colony-stimulating factor
  • hematopoietic growth factor
  • P-cell stimulating factor
  • mast-cell growth factor
  • colony-stimulating factor, multiple
• GenBank acession
  • M14743
• Locus
  5q31.1
• Discovery year
  2001-06-22
• Entrez gene record
  3562
• Pubmed identfication
  • 3489530
• RefSeq identity
  • NM_000588
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000059640

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