IKK alpha/beta (S176 + S180) Antibody

• Catalog number
  bs-3237R
• Price
  Please ask
• Size
  0.1ml

• Long name
  IKK alpha/beta (S176 + S180) Antibody
• Also known as
  IKK alpha/beta (S176 + S180) Antibody
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  IKK alpha/beta Ser176 + Ser180
• Specificity
  This is a highly specific antibody against IKK alpha/beta Ser176 + Ser180. These phosphorylation sites are homologous across the listed species.
• Modification s
  Phosphorylation
• Modification site s
  Ser176 + Ser180
• Clonality
  Polyclonal antibody
• Clone number
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  150-200/756
• Subcellular location
  Nucleus
• Source
  KLH conjugated synthetic phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of Ser176/Ser180
• Gene ID number
  1147
• Swiss Prot
  O15111
• Tested Applications
  WB, IHC-P, IF(IHC-P)
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
• Cross reactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; Nuclear factor NF-kappa-B inhibitor kinase alpha; Transcription factor 16; TCF-16; CHUK
• Description
  The Anti-IKK alpha/beta Ser176 + Ser180 is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  IKK   alpha/beta   S176   S180  
• Gene symbol
  IKBKE, IKBKB, CHUK, IKBKG
• Short name
  Anti-IKK alpha/beta Ser176 + Ser180
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  Immunoglobulin G (IgG)
• Alternative name
  IKK alpha/beta (S176 + S180) Antibody
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:14552
• Gene
  IKBKE
• Long gene name
  inhibitor of nuclear factor kappa B kinase subunit epsilon
• Synonyms gene name
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• Synonyms
  • IKKE
  • IKK-i
  • KIAA0151
• GenBank acession
  • AB016590
• Locus
  1q32.1
• Discovery year
  2002-12-02
• Entrez gene record
  9641
• Pubmed identfication
  • 10421793
  • 10882136
• Classification
  • I kappa B kinases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000184613

Gene info

• Identity
  HGNC:5960
• Gene
  IKBKB
• Long gene name
  inhibitor of nuclear factor kappa B kinase subunit beta
• Synonyms gene name
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Synonyms
  • IKK2
  • NFKBIKB
  • IKK-beta
  • IKKB
• GenBank acession
  • AF029684
• Locus
  8p11.21
• Discovery year
  1998-02-11
• Entrez gene record
  3551
• Pubmed identfication
  • 9878263
  • 9763654
  • 18308615
• Classification
  • I kappa B kinases
  • IKK complex
• VEGA ID
  OTTHUMG00000164092
• Locus Specific Databases
  • LRG_1241

Gene info

• Identity
  HGNC:1974
• Gene
  CHUK
• Long gene name
  component of inhibitor of nuclear factor kappa B kinase complex
• Synonyms gene
  • TCF16
• Synonyms gene name
  • conserved helix-loop-helix ubiquitous kinase
• Synonyms
  • IKK1
  • IKK-alpha
  • IkBKA
  • NFKBIKA
  • IKKA
• Synonyms name
  • inhibitor of nuclear factor kappa-B kinase subunit alpha
  • I-kappa-B kinase
• GenBank acession
  • AF009225
• Locus
  10q24.31
• Discovery year
  1995-03-15
• Entrez gene record
  1147
• Pubmed identfication
  • 7558004
  • 16902410
• RefSeq identity
  • NM_001278
• Classification
  • I kappa B kinases
  • IKK complex
• VEGA ID
  OTTHUMG00000018899

Gene info

• Identity
  HGNC:5961
• Gene
  IKBKG
• Long gene name
  inhibitor of nuclear factor kappa B kinase regulatory subunit gamma
• Synonyms gene
  • IP2
  • IP1
• Synonyms gene name
  • incontinentia pigmenti
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
  • inhibitor of nuclear factor kappa B kinase subunit gamma
• Synonyms
  • IKK-gamma
  • NEMO
  • Fip3p
  • FIP-3
  • FIP3
  • ZC2HC9
  • IKKAP1
  • IKKG
• Synonyms name
  • NF-kappa-B essential modulator
  • IkB kinase-associated protein 1
  • I-kappa-B kinase subunit gamma
  • IkB kinase subunit gamma
  • 14.7K (adenovirus E3 protein) interacting protein 3
• GenBank acession
  • AF074382
• Locus
  Xq28
• Discovery year
  1998-09-30
• Entrez gene record
  8517
• Pubmed identfication
  • 9751060
  • 10087442
  • 11590134
  • 11242109
• RefSeq identity
  • NM_003639
• Classification
  • Zinc fingers C2HC-type
  • IKK complex
• VEGA ID
  OTTHUMG00000024234
• Locus Specific Databases
  • IKBKGbase: Mutation registry for Nemo deficiency
  • Global Variome shared LOVD
  • LRG_70

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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