IgG2b (FITC), IgG2a (PE), Dual Color, Murine Isotype Control[IgG2b]
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Catalog number
MBS655515
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Price
Please ask
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Size
NA
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Products_type
Isotype Control
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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Test
The duality of this product lies in it's dual specificity to the gene, pH or protein. Mainly in phosphorylation by specific phosphatases.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Conjugation
Anti-FITC Antibody
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Group
isotypes
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Gene target
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Short name
IgG2b (FITC), IgG2a (PE), Color, Murine Isotype Control[IgG2b]
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Technique
Control, Isotype, FITC, controls, Fluorescein, Isotypes and controls
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Isotype
IgG2a, IgG2b
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Label
FITC
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Alternative name
IgG2b (fluorecein), IgG2a (peroxidase), Dual Color, Murine isoform reference[IgG2b]
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Alternative technique
control, controls, fluorescine
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Tissue
control
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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