IgG1 antibody conjugated to FITC - IgG2a antibody conjugated to PE - Negative Control

• Catalog number
  G12A
• Price
  Please ask
• Size
  100 Tests

• Category
  Negative Control
• Long description
  Negative control for Immunofluorescence staining with FITC and PE of mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.
• Antibody come from
  n/a
• Other description
  Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
• Clone
  not specified
• Antigen antibody binding interaction
  Mouse IgG1 antibody conjugated to FITC - IgG2a antibody conjugated to PE - Negative Control
• Antibody is raised in
  Mouse
• Antibody s reacts with
  see techfile
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Flow Cytometry
• Antibody s suited for
  Consult the appropriate fact sheet to determine the amount of antibody to be used as a control for PBMC or Whole Blood. PBMC: Gently add10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼ C. Wash twice with PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Storage
  4ºC
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Properties
  If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  IgG1   conjugated   IgG2a   conjugated  
• Short name
  IgG1 antibody conjugated FITC - IgG2a antibody conjugated PE - Negative Control
• Technique
  Antibody, Negative, Control, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, controls, Fluorescein, negatif
• Isotype
  IgG1, IgG1, IgG2a
• Label
  FITC and PE
• Alternative name
  IgG1 (antibody to-) coupled to fluorecein - IgG2a (antibody to-) coupled to peroxidase - Negative reference
• Alternative technique
  controls, antibodies, fluorescine
• Tissue
  control

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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