IgG, Lambda (FITC)

• Catalog number
  MBS626897
• Price
  Please ask
• Size
  5x1 mg

• Products_type
  Antibody
• Products_short_name
  [IgG, Lambda]
• Products_name_syn
  [Anti -IgG, Lambda (FITC)]
• Clonality
  Polyclonal
• Reactivity
  Human
• Specificity
  Recognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
• Purity
  Affinity Purified Purified by immunoaffinity chromatography using human antigens coupled to agarose beads followed by solid phase adsorption.
• Form
  Supplied as a lyophilized powder from PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
• Storage_stability
  Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
• Tested_application
  ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  Lambda  
• Gene symbol
  POLL, CRYL1, IGLC4
• Short name
  IgG, Lambda (FITC)
• Technique
  FITC, IgG, Fluorescein, IgGs
• Host
  Goat
• Isotype
  IgG, IgG
• Label
  FITC
• Alternative name
  Immunoglobulin G, Lambda (fluorecein)
• Alternative technique
  fluorescine, immunoglobulins

Gene info

• Identity
  HGNC:9184
• Gene
  POLL
• Long gene name
  DNA polymerase lambda
• Synonyms gene name
  • polymerase (DNA directed), lambda
  • polymerase (DNA) lambda
• GenBank acession
  • AF161019
• Locus
  10q24.32
• Discovery year
  2000-02-16
• Entrez gene record
  27343
• Pubmed identfication
  • 17686665
• RefSeq identity
  • NM_013274
• Classification
  • DNA polymerases
• VEGA ID
  OTTHUMG00000018933

Gene info

• Identity
  HGNC:18246
• Gene
  CRYL1
• Long gene name
  crystallin lambda 1
• Synonyms gene name
  • crystallin, lambda 1
• Synonyms
  • GDH
  • lambda-CRY
  • MGC149525
  • MGC149526
• Synonyms name
  • crystallin, lamda 1
  • L-gulonate 3-dehydrogenase
  • lambda-crystallin homolog
• GenBank acession
  • AF077049
• Locus
  13q12.11
• Discovery year
  2002-02-22
• Entrez gene record
  51084
• Pubmed identfication
  • 12527201
• RefSeq identity
  • NM_015974
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000016516

Gene info

• Identity
  HGNC:5858
• Gene
  IGLC4
• Long gene name
  immunoglobulin lambda constant 4 (pseudogene)
• Synonyms gene
  • IGLC
• Synonyms gene name
  • immunoglobulin lambda constant 4
  • immunoglobulin lambda constant 4 pseudogene
• GenBank acession
  • J03009
• Locus
  22q11.22
• Discovery year
  1989-05-29
• Entrez gene record
  3540
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151180

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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