IgG, H&L, X-Adsorbed (FITC)

  • Catalog number
    MBS610720
  • Price
    Please ask
  • Size
    1 mg
  • Products_type
    Antibody
  • Products_short_name
    [IgG, H&L, X-Adsorbed]
  • Products_name_syn
    [Anti -IgG, H&L, X-Adsorbed (FITC)]
  • Clonality
    Polyclonal
  • Reactivity
    Mouse
  • Specificity
    Recognizes Mouse IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-donkey serum, mouse IgG and mouse serum. Does not crossreact with bovine, chicken, goat, guinea pig, hamster, equine, human, rabbit, rat and sheep serum proteins.
  • Purity
    Affinity Purified Purified by immunoaffinity chromatography.
  • Form
    Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml 40-50% sterile glycerol, dH2O.
  • Storage_stability
    Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, dH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
  • Tested_application
    ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Gene target
  • Short name
    IgG, H L, X-Adsorbed (FITC)
  • Technique
    FITC, IgG, Fluorescein, IgGs
  • Host
    Donkey
  • Isotype
    IgG, Heavy & Light chains, IgG
  • Label
    FITC
  • Alternative name
    Immunoglobulin G, heavy + light chain, X-Adsorbed (fluorecein)
  • Alternative technique
    fluorescine, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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