IgG, H&L, X-Adsorbed (FITC)
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Catalog number
MBS610720
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Price
Please ask
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Size
1 mg
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Products_type
Antibody
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Products_short_name
[IgG, H&L, X-Adsorbed]
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Products_name_syn
[Anti -IgG, H&L, X-Adsorbed (FITC)]
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Clonality
Polyclonal
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Reactivity
Mouse
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Specificity
Recognizes Mouse IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-donkey serum, mouse IgG and mouse serum. Does not crossreact with bovine, chicken, goat, guinea pig, hamster, equine, human, rabbit, rat and sheep serum proteins.
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Purity
Affinity Purified Purified by immunoaffinity chromatography.
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Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml 40-50% sterile glycerol, dH2O.
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Storage_stability
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, dH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short name
IgG, H L, X-Adsorbed (FITC)
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Technique
FITC, IgG, Fluorescein, IgGs
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Host
Donkey
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Isotype
IgG, Heavy & Light chains, IgG
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Label
FITC
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Alternative name
Immunoglobulin G, heavy + light chain, X-Adsorbed (fluorecein)
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Alternative technique
fluorescine, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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