IDH2 Antibody

• Catalog number
  R32113
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  IDH2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.1-0.5ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  Optimal dilution of the IDH2 antibody should be determined by the researcher.
• Intented use
  This IDH2 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P48735
• Purity
  Antigen affinity
• Description
  Isocitrate dehydrogenase [NADP], mitochondrial is an enzyme that in humans is encoded by the IDH2 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
• Immunogen
  Amino acids KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR of human IDH2 were used as the immunogen for the IDH2 antibody.
• Storage
  After reconstitution, the IDH2 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Cytoplasmic
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  IDH2  
• Gene symbol
  IDH2, IDH2-DT
• Short name
  Anti-IDH2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to IDH2
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:5383
• Gene
  IDH2
• Long gene name
  isocitrate dehydrogenase (NADP(+)) 2
• Synonyms gene name
  • isocitrate dehydrogenase 2 (NADP+), mitochondrial
  • isocitrate dehydrogenase (NADP(+)) 2, mitochondrial
• Locus
  15q26.1
• Discovery year
  1986-01-01
• Entrez gene record
  3418
• Classification
  • Isocitrate dehydrogenases
• VEGA ID
  OTTHUMG00000149815
• Locus Specific Databases
  • LRG_611

Gene info

• Identity
  HGNC:53154
• Gene
  IDH2-DT
• Long gene name
  IDH2 divergent transcript
• Locus
  15q26.1
• Discovery year
  2017-08-03
• Entrez gene record
  105370966
• Classification
  • Divergent transcripts

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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