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Available ordering format
Inquire
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Immunogen
Purified native ICL from Ricinus communis
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Ammonium sulfate purified IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), ELISA (ELISA)
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Connected products
collection of antibodies to proteins involved in carbohydrate metabolism Plant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
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Molecular weight expected аpparent
64.7 | 65 kDa
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Verified reactivity
Ricinus communis
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Possible reactivity
dicots including:Arabidopsis thaliana, Cucumis sativus, Gossypium mexicanum, monocots including: Oryza sativa, trees: Populus balsamifera subsp. trichocarpa
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Manufactured by Operon Biotechnologies.
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References
Maeshima et al. (1988). Evidence for no proteolytic processing during transport of isocitrate lyase into glyoxysomes in castor bean endosperm. Plant Cell Physiol. 29:381-384.
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Scientific context
Isocitrate lyase (EC=4.1.3.1), localized in glyoxysome is an enzyme involved in mobilization of storage lipis during plant seedling growth. Catalalyzed reaction is a conversion of isocitrate to succinate and glyoxylate. Alternative names of this enzyme: isocitrase, isocitritase, isocitratase, glyoxylate-lyase, isocitrate glyoxylatelyase.
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Notes
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.
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Protein number
Refer to NCBI
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TAIR number
AT3G21720
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