ICAM-1 Recombinant Protein
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Catalog number
40-238
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Price
Please ask
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Size
0.01 mg, 0.05 mg
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About recombinant protein
ICAMs are members of the Ig superfamily of calcium-independent transmembrane glycoproteins. ICAM-1 is a ligand for lymphocyte function-associated (LFA) and Mac-1 integrins and the major human rhinovirus receptor. The primary function of ICAM-1 is to provide adhesion between endothelial cells and leukocytes after stress or injury. The human ICAM-1 gene codes for a 505 amino acid transmembrane glycoprotein containing a 29 amino acid cytoplasmic domain, a 23 amino acid transmembrane domain, and a 453 amino acid extracellular domain. Recombinant human ICAM-1 is a 49.5 kDa glycoprotein comprising the extracellular domain (453 amino acid residues) of ICAM-1. Monomeric glycosylated ICAM-1 migrates at an apparent molecular weight of approximately 72.0-80.0 kDa by SDS-PAGE analysis under reducing conditions.
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Other name of the recombinant
BB2, CD54, P3.58, Intercellular adhesion molecule 1, Major group rhinovirus receptor, ICAM-1
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Recombinant in
CHO cells
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SEQUENCE
QTSVSPSKV ILPRGGSVLV TCSTSCDQPK LLGIETPLPK KELLLPGNNR KVYELSNVQE DSQPMCYSNC PDGQSTAKTF LTVYWTPERV ELAPLPSWQP VGKNLTLRCQ VEGGAPRANL TVVLLRGEKE LKREPAVGEP AEVTTTVLVR RDHHGANFSC RTELDLRPQG LELFENTSAP YQLQTFVLPA TPPQLVSPRV LEVDTQGTVV CSLDGLFPVS EAQVHLALGD QRLNPTVTYG NDSFSAKASV SVTAEDEGTQ RLTCAVILGN QSQETLQTVT IYSFPAPNVI LTKPEVSEGT EVTVKCEAHP RAKVTLNGVP AQPLGPRAQL LLKATPEDNG RSFSCSATLE VAGQLIHKNQ TRELRVLYGP RLDERDCPGN WTWPENSQQT PMCQAWGNPL PELKCLKDGT FPLPIGESVT VTRDLEGTYL CRARSTQGEV TRKVTVNVLS PRYE
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Activity
ICAM - 1 supported the adhesion of more than 50% PMA - stimulated HSB2 cells to plates coated at 25 ng/mL ICAM - 1 concentration
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Keep at
The lyophilized ICAM-1 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted ICAM-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing ICAM-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
167466198
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Accession nr
NP_000192.2
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
3383
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Gene name
intercellular adhesion molecule 1
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Source
Homo sapiens
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Uniprot
P05362
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
ICAM1
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Short name
ICAM-1 Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
ICAM-1 Rec. Protein
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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