Human UBE2A(Ubiquitin Conjugating Enzyme E2A) EIA Kit
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Catalog number
E-EL-H2381-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human UBE2A. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBE2A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBE2A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human UBE2A. You can calculate the concentration of Human UBE2A in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.19ng/mL
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Detection Range
0.31~20ng/mL
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Target Name
UBE2A
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UNIProt ID
P49459
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human UBE2A(Ubiquitin Conjugating Enzyme E2A) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbol
UBE2A, TCF3
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Short name
UBE2A(Ubiquitin Conjugating Enzyme E2A) EIA Kit
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Technique
Enzyme, EIA, eia, enzymes
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Species
Human, Humans
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Alternative name
H. sapiens UBE2A(Ubiquitin Conjugating Enzyme E2A) EIA reagent
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Alternative technique
enzymes, kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
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Identity
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Gene
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Long gene name
transcription factor 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1990-07-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic helix-loop-helix proteins
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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